This paper proposes a resource-aware performance diagnostic method. For transactions in Web applications, the proposed method constructs performance profile chains based on the resource service time, which is stable for different workload characteristics. According to the anomaly of resource service time at application runtime, the proposed method provides an efficient solution to performance anomaly detection, location and diagnosis. Experimental results show that this method can effectively detect performance anomalies caused by different resource bottlenecks with changing workload characteristics.