The chain measurement mechanism of trusted computing doesn’t easily extend to all applications in the terminal, so it is difficult for the terminal to always maintain the trust of the dynamic running environment of the terminal. To collect trustworthiness evidence in an objective, genuine, and comprehensive way, a trusted evidence collection agent based on TPM is designed and developed. Its main function is collecting the critical objects in the dynamic environment of the terminal, such as memory, process, disk files, network ports, policy data, and so on. First, the static and dynamic creditability of the agent is assured by the measurement function of trusted platform module (TPM) and isolation mechanism of trusted virtual machine monitor (TVMM), and then the creditability of original and transmit of the collecting evidences is assured by the encryption and signature function. This paper also implements a prototype of the agent in Windows platform. Based on the prototype, the paper examines the trustworthiness evaluation for executing the agent program in a local area network distributed computing environment. In this application, the performance of prototype is studied, and the feasibility of this approach is demonstrated.