Cell’s immunological information is acquired through analyzing color immunofluorescence image. A pixel growing algorithm with dynamic seed values is proposed to extract fluorescent regions in the CIE (L*, a*, b*) uniform color space. Ellipse matching method is proposed to extract cells from the obtained fluorescent regions. Finally antigen information is acquired through computing and decomposing color of the cell with the pre-computation and discretization method. The methods proposed for the acquirement of cell’s immmunological information are appropriately evaluated. The experiment indicates that acquired the immunological information meets practical medicine analysis.