Watermarking technique is a method by hiding copyright information into covering signals to discourage unauthorized copying. Because the profiles of two-dimension Gabor base functions are similar to those of human visual cortical cell receptive field and the middle frequency of visual channels has octave relationship, a video watermarking algorithm is proposed based on spatio-temporal multi-channel model using 3D Gabor transform in this paper. Experimental results indicate that the Gabor domain watermarks have greater robustness and imperceptibility.